1.Preparation phase

Determine the filtering target: Clearly identify the types and quantities of bacteria that need to be filtered, as well as the standards that need to be achieved after filtration.

Select filtration equipment: Choose the appropriate filtration equipment based on the filtration target, such as microporous membrane filters, deep layer filters, or centrifugal filters. These devices each have their own advantages and disadvantages, and should be selected according to actual needs.

Preparation of culture medium and reagents: For microbiological testing, it is necessary to prepare appropriate culture media (such as nutrient agar, rose red sodium agar, etc.) as well as relevant flushing and dilution solutions.

2.Sample processing

Sampling: Take an appropriate amount of sample from the sample to be filtered, taking care to avoid contamination.

Dilution: If the bacterial concentration in the sample is high, appropriate dilution is required for better filtration and counting.

3.Filtering operation

Preprocessing: Clean and sterilize the filtering equipment and related vessels to ensure a sterile environment.

Filtering: Filter the processed sample through a filtering device. During the filtration process, it may be necessary to add an appropriate amount of flushing solution to remove any residue on the filter membrane.

Collect filter membrane: After filtration is completed, remove the filter membrane from the filtration equipment and pay attention to maintaining its sterile state.

4.Cultivation and observation

Inoculation: Place the filter membrane bacteria face up onto the prepared culture medium for inoculation.

Cultivation: Set appropriate cultivation temperature, humidity, and time based on the growth conditions of the target bacteria. Bacteria usually need to be cultured for a certain period of time (such as 48 hours) to form visible colonies.

Observation: Regularly observe the growth of colonies on the culture medium during the cultivation process, and record information such as the number, morphology, and distribution of colonies.

5.Result analysis and reporting

Counting: Calculate the number of bacteria in the sample based on the growth of bacterial colonies. Pay attention to following the relevant principles for reporting bacterial count.

Result analysis: Compare and analyze the counting results with the preset standards to evaluate whether the filtering effect meets the standards.

Report writing: Write a detailed report based on the experimental results, including information on experimental methods, experimental conditions, experimental results, and conclusions.

It should be noted that the process of biological bacterial filtration may vary depending on specific application scenarios. In practical operation, adjustments and optimizations should be made according to specific needs and actual situations. At the same time, strict adherence to sterile operating procedures should be followed throughout the entire process to ensure the accuracy and reliability of experimental results.